Report under the Scientific Cooperation Agreement with the Autonomous Non-Commercial Organization “SFERA” — Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, 2025
Overview
This 2025 study from the Pavlov Institute of Physiology (IFRAN) investigates the effects of a latest-generation WiFi6 router and Lifetune Zone Max resonators on behavior and blood parameters in rats with contrasting nervous system excitability. Conducted in a Faraday cage to control for background electromagnetic fields, the study found that router EMR altered both behavioral and hematological parameters in both rat strains — and that Lifetune Zone Max resonators restored these parameters to control levels in both strains.
This is the most recent study in the IFRAN rat research series, updating the 2018–2020 work with current-generation WiFi6 technology and the latest Aires resonator product.
Institution and Authors
Institution: Federal State Budgetary Institution of Science, Pavlov Institute of Physiology, Russian Academy of Sciences (IF RAN), Higher Nervous Activity Laboratory
Responsible Executors: PhD. Shalaginova I.G.; PhD. Pavlova M.B.
Location: St. Petersburg, Russia
Year: 2025
Study Design
Three experimental conditions:
- Control (C) — vivarium conditions, no exposure
- Router alone (R) — 4 days, 12 hours/day WiFi6 exposure
- Router + Resonators (R+D) — 4 days, 12 hours/day WiFi6 with 6 Lifetune Zone Max resonators (one per side of Faraday cage)
Router: TP-Link Archer AX73, AX5400 dual-band Gigabit WiFi6, 1.5 GHz 3-core processor, 6 antennas with Beamforming
Animals: 5-month-old male rats, two contrasting strains: HT (low-excitability) and LT (high-excitability)
Behavioral tests: Open Field (OF), Elevated Plus Maze (EPM), Light-Dark Chamber (LDC)
Blood analysis: 21 hematological parameters (before and after 4-day exposure)
Results — Behavioral Parameters
HT Strain (low-excitability)
- Open Field: Router increased horizontal and vertical motor activity (ANOVA p = 0.0023 / p = 0.0055). With resonators: returned to control levels.
- Dark-Light Chamber: Router decreased latency and increased light-chamber time (p = 0.0028 / p = 0.0015). With resonators: comparable to control.
- Elevated Plus Maze: No changes.
LT Strain (high-excitability)
- Open Field: Router increased vertical motor activity (ANOVA p = 0.0264). With resonators: returned to control level.
- Elevated Plus Maze and Dark-Light Chamber: No significant changes.
Results — Blood Parameters
HT Strain
- Router decreased hemoglobin; resonators normalized hemoglobin concentration
LT Strain (high-excitability) — strongest effects
- Router increased hematocrit → resonators normalized hematocrit (Kruskal-Wallis p = 0.009)
- Router increased granulocytes → resonators normalized granulocyte % (Kruskal-Wallis p = 0.031)
- Router decreased lymphocytes → resonators normalized lymphocytes (p = 0.025)
- Router increased red blood cell count → resonators normalized RBC count (Kruskal-Wallis p = 0.013)
- Router increased hemoglobin → resonators normalized hemoglobin to control
Conclusions
1. “The results of this study confirmed the greater susceptibility of high-excitability rats of the LT strain to the influence of router EMR.”
2. “For blood parameters, genotype-dependent changes after exposure to router radiation have been demonstrated for the first time.”
3. “A recovery in behavioral activity and blood parameters in both strains was demonstrated through the use of resonators under router operating conditions.”
“The results of this study confirm the negative impact on the behavior and hematological parameters of rats of UHF EMF from a standard latest-generation router and demonstrate the positive effect of new-generation resonators on the restoration of a number of behavioral elements and blood parameters affected by non-ionizing electromagnetic radiation.”
Context: 2018–2020 Program Continuation
This study continues the IFRAN research program begun under the Aires Human Genome Research Foundation cooperation agreement. Prior IFRAN studies with Aires Defender Pro and 2.4 GHz WiFi established behavioral normalization and reduced chromosomal aberrations (Dyuzhikova et al., 2019). The 2025 study updates to WiFi6 (1.5 GHz) and Lifetune Zone Max, finding the same normalization pattern across behavioral and hematological parameters in a more challenging exposure environment.
Why This Study Matters
The two-strain design is a methodological strength: LT and HT rats are genetically selected for contrasting nervous system excitability, so differences in response reflect biological variation, not measurement noise. The finding of genotype-dependent blood parameter changes — normalized by resonators that also produce opposite changes in hemoglobin direction between strains — is inconsistent with a non-specific or placebo response and requires a biological mechanism.
Full Citation
Shalaginova I.G., Pavlova M.B. “Study of the effect of non-ionizing electromagnetic radiation from the Archer AX73 WiFi6 router and Lifetune Zone Max resonators on the behavior and blood parameters of rats with different nervous system excitability.” Report under the Scientific Cooperation Agreement with ANO “SFERA.” Pavlov Institute of Physiology, Russian Academy of Sciences. St. Petersburg, 2025.
Related IFRAN Animal Research
IFRAN Bee Study 2016 — WiFi altered honeybee behavior; Aires Defender normalized it
IFRAN Bee Stage 5, 2019 — Heat shock protein (HSP70) expression normalized by Aires resonator
Dyuzhikova et al. 2019 — Chromosomal aberrations and nervous system excitability in Wistar rats
PACE Bee Field Study 2023 — Field conditions, bee behavior, Aires resonator
→ Animal Research Overview | → Why Aires Research Is Different